Instruments

Thermo LTQ Orbitrap XL Mass Spectrometer

• Resolving power (100,000)
• Mass range m/z=15–4,000 amu
• Mass accuracy (<1 ppm with internal calibration)
• Sensitivity (sub femtomol)
• Dynamic range (>4,000)
• Fragmentation methods (CID, PQD, HCD)
• Ion sources (ESI, APCI, APPI)
•     Modified for studies of SCMS, MSI, and live spheroids

Waters nanoACQUITY 2D UPLC

• Featured 2D Reverse Phase/Reverse Phase (RP/RP) separation
• High-resolution in both dimensions
• Improved protein identifications, quantification, and sequence coverage
• Coupled to Thermo LTQ Orbitrap XL mass spectrometer

Thermo TSQ Quantiva Triple Quad Mass Spectrometer

• Mass range m/z=10–1,800 amu
• Six orders of dynamic range
• Ion Sources (HESI, APCI)
• Modified for studies of ion–molecule reactions

The Single-probe MS Devices (2)

• Coupled with Thermo LTQ Orbitrap XL mass spectrometers
• Used for SCMS, MSI, and live spheroids experiments
• Equipped with two digital microscopes
• Computer-controlled XYZ-stage (0.1 μm increment)
• Can be potentially customized for other MS systems

Integrated cell manipulation system

• Include two Eppendorf TransferMan stages, a micro-injector, a high-resolution inverted microscope, a thermoplate, a cell selection probe, and a Single-probe
• Coupled to Thermo LTQ Orbitrap XL mass spectrometers
• Used for selection of suspension single cells (e.g. patient cells in solutions) for MS measurement
• Cell size can be measured prior to MS analysis

Thermo UltiMate 3000 RSLCnano LC

• Flow delivery from 20 nL/min up to 50 µL/min at a maximum pressure of 800 bar
• High-precision sample injections down to 10 nL
• High flexibility for customized designs of experiment setups
• Coupled to Thermo TSQ Quantiva Triple Quad Mass Spectrometer